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Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA)

Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP433314BONYeast1mgQuote
EP433314BONE. coli1mgQuote
BP433314BONBaculovirus200ugQuote
MP433314BONMammalian Cell200ugQuote

Protein Information

SpeciesBacillus weihenstephanensis (strain KBAB4)
UniProt IDA9VRG6
Gene NamerocA; Locus:BcerKBAB4_0290
Protein Name1-pyrroline-5-carboxylate dehydrogenase
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMVVAYKHEPFTDFSVEANKLAFEEGLKKVESYLGQDYPLIIGGEKITTEDKIVSVNPANK EELVGRVSKASRELAEKAMQVADATFQTWRKSKPEMRADILFRAAAIVRRRKHEFSAILV KEAGKPWNEADADTAEAIDFMEYYARQMLKLKDGMPVESRPIEYNRFSYIPLGVGVIISP WNFPFAIMAGMTTAAVVSGNTVLLKPASTTPVVAAKFMEVLEEAGLPAGVVNFVPGNGSE VGDYLVDHPRTRFVSFTGSRDVGIRIYERAAKVNPGQIWLKRVIAEMGGKDTIVVDKEAD LELAAKSIVASAFGFSGQKCSACSRAVIHEDVYDHVLNRAVELTKELTVGNPDAKDINMG PVNDQAAFDKVMSYVAIGKEEGKIVSGGEGDDSKGWFIQPTIVADVAEDARLMKEEIFGP VVAFCKAKDFDHALAIANNTEYGLTGAVLSNNRDHIEKAREDFHVGNLYFNRGCTGAIVG YQPFGGFNMSGTDSKAGGPDYLALHMQAKTTSETL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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