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Recombinant Pyrimidine monooxygenase RutA (rutA)

Recombinant Pyrimidine monooxygenase RutA (rutA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514895EOFYeast1mgQuote
EP514895EOFE. coli1mgQuote
BP514895EOFBaculovirus200ugQuote
MP514895EOFMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli O157:H7 (strain TW14359 / EHEC)
UniProt IDC6UPN4
Gene NamerutA; Locus:ECSP_1181
Protein NamePyrimidine monooxygenase RutA
Region Expressed1-382
Expression Tag6xHis
Purity>90%
AA SequenceMQNAAPRLTFTLRDEERLMMKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYH FDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMA ATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKSD FKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFAPTA ARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRS GTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETF GERIQPLMQCRAHLPVLTQEVA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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