The Parvalbumin can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For fluid-phase adsorption in immunohistochemistry we suggest the following procedure: A- Dilute 1 ul of the monoclonal antibody 235 against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). B- Add 1ug of the Parvalbumin to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. D- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.
The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris-base, 70mM NaCl, 1mM EDTA and 1mM b-mercaptoethanol, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 90% as determined by SDS-PAGE.
It is recommended to reconstitute the lyophilized Parvalbumin in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Parvalbumin although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Parvalbumin should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Pvalb, Pva, PALB1, Parvalbumin.
