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Recombinant Pumilio homology domain family member 4 (PUF4)

Recombinant Pumilio homology domain family member 4 (PUF4) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP340691SVGYeast1mgQuote
EP340691SVGE. coli1mgQuote
BP340691SVGBaculovirus200ugQuote
MP340691SVGMammalian Cell200ugQuote

Protein Information

SpeciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
UniProt IDP25339
Gene NamePUF4; Locus:YGL014W; ORFs:YGL023
Protein NamePumilio homology domain family member 4
Region Expressed1-888
Expression Tag6xHis
Purity>90%
AA SequenceMSTKGLKEEIDDVPSVDPVVSETVNSALEQLQLDDPEENATSNAFANKVSQDSQFANGPP SQMFPHPQMMGGMGFMPYSQMMQVPHNPCPFFPPPDFNDPTAPLSSSPLNAGGPPMLFKN DSLPFQMLSSGAAVATQGGQNLNPLINDNSMKVLPIASADPLWTHSNVPGSASVAIEETT ATLQESLPSKGRESNNKASSFRRQTFHALSPTDLINAANNVTLSKDFQSDMQNFSKAKKP SVGANNTAKTRTQSISFDNTPSSTSFIPPTNSVSEKLSDFKIETSKEDLINKTAPAKKES PTTYGAAYPYGGPLLQPNPIMPGHPHNISSPIYGIRSPFPNSYEMGAQFQPFSPILNPTS HSLNANSPIPLTQSPIHLAPVLNPSSNSVAFSDMKNDGGKPTTDNDKAGPNVRMDLINPN LGPSMQPFHILPPQQNTPPPPWLYSTPPPFNAMVPPHLLAQNHMPLMNSANNKHHGRNNN SMSSHNDNDNIGNSNYNNKDTGRSNVGKMKNMKNSYHGYYNNNNNNNNNNNNNNNSNATN SNSAEKQRKIEESSRFADAVLDQYIGSIHSLCKDQHGCRFLQKQLDILGSKAADAIFEET KDYTVELMTDSFGNYLIQKLLEEVTTEQRIVLTKISSPHFVEISLNPHGTRALQKLIECI KTDEEAQIVVDSLRPYTVQLSKDLNGNHVIQKCLQRLKPENFQFIFDAISDSCIDIATHR HGCCVLQRCLDHGTTEQCDNLCDKLLALVDKLTLDPFGNYVVQYIITKEAEKNKYDYTHK IVHLLKPRAIELSIHKFGSNVIEKILKTAIVSEPMILEILNNGGETGIQSLLNDSYGNYV LQTALDISHKQNDYLYKRLSEIVAPLLVGPIRNTPHGKRIIGMLHLDS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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