Protein A/G Recombinant
The recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C2 and C3. The fusion protein has a predicted molecular mass of 50.5 kDa. But it migrates with an apparent molecular mass of approximately 45kDa in SDS-PAGE.
Accession
Applications
Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone, occurring well at pH 5-8.
Formulation
Lyophilized white Powder containing no additives.
Purity
>95% as determined by SDS-PAGE and RP-HPLC. A280 of 0.1% solution~0.505.
Reconstitution
Reconstitution with deionized water or PBS.
Source
Storage
2 years at -20oC. After reconstitution, aliquot and store at -20oC.
Avoid repeated freeze/thaw cycles.
