Request QuoteCatalog Number: xP508736BWLSize: 0.2-1mg

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Recombinant Proline--tRNA ligase (proS)

Recombinant Proline--tRNA ligase (proS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP508736BWLYeast1mgQuote
EP508736BWLE. coli1mgQuote
BP508736BWLBaculovirus200ugQuote
MP508736BWLMammalian Cell200ugQuote

Protein Information

SpeciesBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
UniProt IDC0ZF57
Gene NameproS; Locus:BBR47_34390
Protein NameProline--tRNA ligase
Region Expressed1-581
Expression Tag6xHis
Purity>90%
AA SequenceMKQSQMLIPTLREVPSDAEIASHKLLLRAGMARQLASGIYTYLPLALRSLHKIQAIVREE MNNAGGQELLMPAMQPAELWHQTGRWDVYGPELVRLRDRHDRPFALGPTHEEVITSLVRD EINSYKKLPINLYQIQTKFRDEVRPRFGLIRCREFIMKDAYSFDTSQDGLDRNFQAMYDA YTNIFTRVGLNFRAVEADAGAIGGKGTYEFMALCDIGEDTIAYSEEGDFAANLEKAEVVY KPSAKPATEAPAREKLHTPGIKTIDQLVQALQIEAKQIIKSLIYRVDDKLVMVLVRGDHE INEVKLKNLYDATIVGLASEADIVSVTGAPAGFVGPVGIDPEKVDIIADNFVQDVYDGVV GANETDYHLTHVVAGRDFTVSKYADLRNITEGDECPRTGGVIKFARGVEVGHVFKLGTKY STAMGATYLDENGRSQPMIMGCYGIGVSRTIAAVIEQNNDENGIIWPVSVAPFHVHVIPV NVKVEEQRQASEQITEALRKAGIEVLFDDRPERAGVKFKDADLIGLPLRITVSDKAAQEG TVEVRIRKNGETHDVKLDQLVDEVKGILSRVDQTGAALFGN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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