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Recombinant Probable 3-beta-hydroxysteroid-Delta (8) ,Delta (7) -isomerase

Recombinant Probable 3-beta-hydroxysteroid-Delta (8) ,Delta (7) -isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP526177DOAYeast1mgQuote
EP526177DOAE. coli1mgQuote
BP526177DOABaculovirus200ugQuote
MP526177DOAMammalian Cell200ugQuote

Protein Information

SpeciesArabidopsis thaliana (Mouse-ear cress)
UniProt IDO48962
Gene NameLocus:At1g20050; ORFs:T20H2.18
Protein NameProbable 3-beta-hydroxysteroid-Delta (8) ,Delta (7) -isomerase
Region Expressed1-223
Expression Tag6xHis
Purity>90%
AA SequenceMEELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGRKKAKLDKLLMC WWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGITA VIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYSY YIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETKTKKKTR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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