$678.00Catalog Number: BXJ1105-wtSize: 20 ug

pMEVHA-JNK1-WT

Human JNK1 (MAPK8) Xpress Clones

Mitogen-activated protein kinases (MAPKs) cascade relays extracellular signals from cell membrane to the nucleus to induce intracellular responses and to regulate many aspects of cell physiology. These cascades, including JNK, ERK and p38 pathways, consist of distinct members of regulatory enzymes that serially activate one another in response to growth factors, cytokines and other mitogenic stimuli, leading to (in)activation of transcription factors. Activation by external stimuli like UV of the Jun N-terminal Kinase (JNK) pathway increases the expression of a set of autoimmune and inflammatory genes, including IL-2 and gamma interferon. Multiple subtypes of the enzyme induce cell- and stimulus- specific responses. The mutation K55M renders the enzyme catalytically inactive.

Includes and available separately

BXJ1105-4:20 ug each
BXJ1105-wt:20 ug
BXJ1105-km:20 ug
BXJ1105-ee:20 ug
BXJ1105-aa:20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Bam HI
3' cloning site:Kpn I
GenBank#:NM_002750
Mutations:K55M; T183E, Y185E; T183A, Y185A

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

Human JNK1 protein sequence

Human JNK1 nucleotide sequence

About Xpress Clones

Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.

Quantity

$678.00

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