$678.00Catalog Number: BXN1001-kaSize: 20 ug

pMEV2HA-NIK-KKAA

Human NIK Xpress Clones

NIK (for NFkB-inducing kinase) is a serine/threonine protein kinase of 947-amino acid. The gene was originally from a B-cell cDNA library. The gene was later renamed as mitogen-activated protein kinase kinase kinase 14 after the emergence of many kinases involved in the MAP kinase cascade.

NIK binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptors.

K429 is catalytically essential, and hence K429/430A mutant is kinase-deficient and can be used to inhibit the NIK activity in cells (i.e. dominant negative). NIK will be activated when T559 is phosphorylated. Alanine substitution of T559 abolishes its activity and its ability to phosphorylate and activate IKKalpha. T559A mutant also dominantly interferes with TNF-a induction of NF-kB, presumably by quenching its upstream activators.

Gene Name: Homo sapiens mitogen-activated protein kinase kinase kinase 14 (MAP3K14); AKA: NFkB-Inducing Kinase (NIK); Serine/Threonine Protein Kinase NIK (HSNIK); HS, FTDCR1B.

Includes and available separately

BXN1001-3:20 ug each
BXN1001-wt:20 ug
BXN1001-ka:20 ug
BXN1001-ta:20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Bam HI
3' cloning site:Kpn I
GenBank#:NM_003954
Mutations:K429A, K430A; T559A

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

Human NIK protein sequence

Human NIK nucleotide sequence

About Xpress Clones

Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.

Quantity

$678.00

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