$678.00Catalog Number: BXH1012-krSize: 20 ug

pMEV2HA-MST2-K56R

Human MST2 Xpress Clones

MST2 is a serine/threonine kinase originally identified from a HeLa cell (Creasy and Chernoff, 1995) and Jurkat (Taylor et al., 1996) cDNA libraries. Yeast "sterile 20" (Ste20) kinase is MST2's homolog and acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. In mammalian cells, MST2 activated by the proapoptotic agents straurosporine and FAS ligand (Taylor et al., 1996; Lee et al., 2001). Gene knock out experiments indicated that MST2 activation is associated with apoptosis (O'Neil et al., 2004). MST2, and it's homolog in Drosophila (hippo, Hpo kinase), is a part of the Hippo kinase cascade that was recently shown to promote cell proliferation and inhibits apoptosis (Zhao et al., 2008; Dong et al., 2007, Harvey et al., 2003).

MST2 protein contains 491-amino acid and can phosphorylate myelin basic protein (MBP), histone H1, and itself. The catalytic domain of MST2 is at the N-terminus of the protein. Lysine residue 56 is a catalytically essential residue, and hence, mutating it to arginine abolishes the kinase activity of MST2. Therefore, the K56R-expressing vector can be used as a dominant negative to inhibit Hippo pathway in mammalian cells.

Gene Name: Homo sapiens Serine/Threonine Kinase 3 (STK3); AKA: MST-2, mammalian serine/threonine kinase 3 (STK3); STE20-like kinase MST2; mammalian STE20-like protein kinase 2; serine/threonine-protein kinase Krs-1.

Includes and available separately

BXH1012-2:20 ug each
BXH1012-wt:20 ug
BXH1012-kr:20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Bam HI
3' cloning site:Xba I
GenBank#:NM_006281
Mutations:K56R

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

Human MST2 protein sequence

Human MST2 nucleotide sequence

Select references

  • Creasy, C. L., et al., 1995. Gene 167: 303-306
  • Dong J. et al., 2007, Cell, 130: 1120-1133
  • Harvey, K. F., et al., 2003. Cell 114: 457-467
  • Lee, K.-K., et al., 2001. J. Biol. Chem. 276: 19276-19285
  • O'Neil E. et al., 2004. Science 306: 2267-2270
  • Taylor, L. K., et al., 1996. Proc. Nat. Acad. Sci. 93: 10099-104
  • Zhao B. et al., 2007. Genes Dev., 21: 2747-61

About Xpress Clones

Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.

Quantity

$678.00

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