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$816.00Catalog Number: BXP1055-wtSize: 20 ug

pMEV2HA-IRAK4-WT

Human IRAK4 Xpress Clones

Interleukin-1 receptor-associated kinases (IRAK), identified as a serine/threonine kinases coprecipitated with IL-1 receptor (IL-1R) in an IL-1 inducible manner, share significant homology to pelle. The mammalian family of IRAK consists of two active kinases, IRAK-1 and IRAK-4; and two inactive kinases, IRAK-2 and IRAK-M. They are implicated in the TLR/IL-1R pathway to both IKK and MAPKs activation, leading to the activation of transcription factors like NF-kB and AP-1.

Human IRAK-4 gene (AF445802) encodes a protein of 52kDa, linked to Ptk9. Northern blots show its expression predominantly in kidney and liver samples, although RT-PCR show low level expression in multiple tissues. Like other IRAKs, IRAK-4 is a multidomain protein, consisting of N-terminal death domain (DD) and a central kinase domain (KD) but lacks the long uniques C-terminal domain. Mutation in the ATP binding pocket, KK213AA renders the enzyme catalytically inactive. In contrast to kinase deficient IRAK-1-KD, which is still able to activate NF-kB and does not inhibit IL-1-dependent signaling, kinase deficient IRAK-4-KK213AA strongly diminished IL-1-induced NF-kB activation suggesting a biological function of the kinase domain in the signal transduction pathways.

Gene Name: Homo sapiens interleukin-1 receptor-associated kinase 4.

Includes and available separately

BXP1055-2:20 ug each
BXP1055-wt:20 ug
BXP1055-ka:20 ug

Custom size is also available upon request.

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Bam HI
3' cloning site:Bam HI
GenBank#:AF445802
Mutations:KK213AA
Quantity

$816.00

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