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$816.00Catalog Number: BXP1050-kaSize: 20 ug

pMEV2HA-IRAK1-KA

Human IRAK1 Xpress Clones

Interleukin-1 receptor-associated kinases (IRAK), identified as a serine/threonine kinases coprecipitated with IL-1 receptor (IL-1R) in an IL-1 inducible manner, share significant homology to pelle. The mammalian family of IRAK consists of two active kinases, IRAK-1 and IRAK-4; and two inactive kinases, IRAK-2 and IRAK-M. They are implicated in the TLR/IL-1R pathway to both IKK and MAPKs activation, leading to the activation of transcription factors like NF-κB and AP-1.

Human IRAK-1 gene (NM_001569) encodes a protein of 90kDa in a wide variety of tissues Like other IRAKs, IRAK-1 is a multidomain protein, consisting of N-terminal death domain (DD) and a central kinase domain (KD). Mutation in the ATP binding pocket, K239A renders the enzyme catalytically inactive. Overexpression of the kinase deficient IRAK-1 did not interfere with IL-1-induced NF-kB activation but strongly induced NF-kB activation on its own. The kinase activity of IRAK-1 is complementary to kinase activity lacking IL-1Rs or TLRs to transduce their signals through homophilic interaction of TIR and DD domain.

Gene Name: Homo sapiens interleukin-1 receptor-associated kinase 1.

Includes and available separately

BXP1050-2:20 ug each
BXP1050-wt:20 ug
BXP1050-ka:20 ug

Custom size is also available upon request.

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Bam HI
3' cloning site:Bam HI
GenBank#:NM_001569
Mutations:K239A
Quantity

$816.00

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