$678.00Catalog Number: BXW1000-dnSize: 20 ug

pMEV2HA-IkBa-DN

Human IκB alpha Xpress Clones

I kappa B proteins (IκBs) bind to and inhibit the NF-κB complex by trapping it in the cytoplasm (OMIM #164008). IkBs could be phosphorylated by IKKs and subsequently polyubiquitinated for degradation to allow translocation of NF-κB into the nucleus. Serines 32 and 36 in the N terminal regulatory domain of IkBa are the conserved phosphorylation sites. Substitution with alanines (S32A and S36A) of the phosphoacceptor sites would block the phosphorylation and degradation of IkBa by IKKs.

Gene Name: Homo sapiens I kappa B alpha (IκBα).

Includes and available separately

BXW1000-2:20 ug each
BXW1000-wt:20 ug
BXW1000-dn:20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Bam HI
3' cloning site:Xba I
GenBank#:NM_020529
Mutations:DN: S32A and S36 A

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

Human IkBa protein sequence

Human IkBa nucleotide sequence

About Xpress Clones

Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.

Quantity

$678.00

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