$678.00Catalog Number: BXP1010-gvSize: 20 ug

pMEV2HA-HRAS-G12V

Human Ha-Ras Xpress Clones

Ras proteins are members of small GTPase superfamily. They bind GDP/GTP and possess intrinsic GTPase activity. These proteins act as switches to turn on/off their downstream target proteins in response to upstream stimuli because they can alternate between an inactive form bound to GDP and an active form bound to GTP. Activating mutations of HRAS, including G12V, activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors. Defects in HRAS are also the cause of oral squamous cell carcinomas (oscc). S17N is a widely used dominant negative mutant of HRAS.

Gene Name: Homo sapiens v-Ha-ras Harvey rat sarcoma viral oncogene homolog. Other Names: HRAS1, RASH1, Ha-Ras

Includes and available separately

BXP1010-3:20 ug each
BXP1010-wt:20 ug
BXP1010-sn:20 ug
BXP1010-gv:20 ug

Custom size is also available upon request.

Specifications

Materials Provided:20 ug transfection ready plasmids in 40ul TE buffer.
Antibiotic Selections:Kan/Neo for kanamycin selectin in bacteria and G418 selection in mammalian cells
Cloning vector:pMEV-2HA
5' cloning site:Bam HI
3' cloning site:Eco RI
GenBank#:NM_005343
Mutations:S17N; G12V

Maintenance of plasmid clones

Upon receiving of the plasmids, briefly spin the vials to collect contents to the bottom before storing at 2-8oC if will be used shortly, or -20 oC for long term storage. It is recommended that you propagate the vectors in E. coli strains that are recombinant deficient (recA) and endonuclease A-deficient (endA) (e.g. DH5α or XL1-Blue).

Human Ha-Ras protein sequence

MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY
RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC
VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL
AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV
REIRQHKLRK LNPPDESGPG CMSCKCVLS

Human Ha-Ras nucleotide sequence

ATGACGGAAT ATAAGCTGGT GGTGGTGGGC GCCGGCGGTG TGGGCAAGAG
TGCGCTGACC ATCCAGCTGA TCCAGAACCA TTTTGTGGAC GAATACGACC
CCACTATAGA GGATTCCTAC CGGAAGCAGG TGGTCATTGA TGGGGAGACG
TGCCTGTTGG ACATCCTGGA TACCGCCGGC CAGGAGGAGT ACAGCGCCAT
GCGGGACCAG TACATGCGCA CCGGGGAGGG CTTCCTGTGT GTGTTTGCCA
TCAACAACAC CAAGTCTTTT GAGGACATCC ACCAGTACAG GGAGCAGATC
AAACGGGTGA AGGACTCGGA TGACGTGCCC ATGGTGCTGG TGGGGAACAA
GTGTGACCTG GCTGCACGCA CTGTGGAATC TCGGCAGGCT CAGGACCTCG
CCCGAAGCTA CGGCATCCCC TACATCGAGA CCTCGGCCAA GACCCGGCAG
GGAGTGGAGG ATGCCTTCTA CACGTTGGTG CGTGAGATCC GGCAGCACAA
GCTGCGGAAG CTGAACCCTC CTGATGAGAG TGGCCCCGGC TGCATGAGCT
GCAAGTGTGT GCTCTCCTGA

About Xpress Clones

Xpress Clones are a collection of expression verified genes, including protein kinase genes, dominant negative mutants, consititutively active mutants and kinase deficient mutants, and other expression ready genes. Unlike other collections aiming to have more genes, we focus on the genes important to your research, the mutations with a meaning to your experiment.
If your gene of interest is not in our list, please let us know and we can clone the genes with desired mutant forms, expression verified in the organism you choose.

Quantity

$678.00

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