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Recombinant 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)

Recombinant 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP501848VEZYeast1mgQuote
EP501848VEZE. coli1mgQuote
BP501848VEZBaculovirus200ugQuote
MP501848VEZMammalian Cell200ugQuote

Protein Information

SpeciesVibrio cholerae serotype O1 (strain M66-2)
UniProt IDC3LN54
Gene NamearoA; Locus:VCM66_1672
Protein Name3-phosphoshikimate 1-carboxyvinyltransferase
Region Expressed1-426
Expression Tag6xHis
Purity>90%
AA SequenceMESLTLQPIELISGEVNLPGSKSVSNRALLLAALASGTTRLTNLLDSDDIRHMLNALTKL GVNYRLSADKTTCEVEGLGQAFHTTQPLELFLGNAGTAMRPLAAALCLGQGDYVLTGEPR MKERPIGHLVDALRQAGAQIEYLEQENFPPLRIQGTGLQAGTVTIDGSISSQFLTAFLMS APLAQGKVTIKIVGELVSKPYIDITLHIMEQFGVQVINHDYQEFVIPAGQSYVSPGQFLV EGDASSASYFLAAAAIKGGEVKVTGIGKNSIQGDIQFADALEKMGAQIEWGDDYVIARRG ELNAVDLDFNHIPDAAMTIATTALFAKGTTAIRNVYNWRVKETDRLAAMATELRKVGATV EEGEDFIVITPPTKLIHAAIDTYDDHRMAMCFSLVALSDTPVTINDPKCTSKTFPDYFDK FAQLSR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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