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Recombinant Phosphoribosylformylglycinamidine cyclo-ligase (purM)

Recombinant Phosphoribosylformylglycinamidine cyclo-ligase (purM) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP513337ENUYeast1mgQuote
EP513337ENUE. coli1mgQuote
BP513337ENUBaculovirus200ugQuote
MP513337ENUMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12 / MC4100 / BW2952)
UniProt IDC4ZX74
Gene NamepurM; Locus:BWG_2263
Protein NamePhosphoribosylformylglycinamidine cyclo-ligase
Region Expressed1-345
Expression Tag6xHis
Purity>90%
AA SequenceMTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGGFGALCALPQKYREPVL VSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEPLFFLDYYATGKLDVDTAS AVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFCVGVVEKSEIIDGSKVSDGDV LIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPLADHLLAPTRIYVKSVLELIEKV DVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQWPEVFNWLQTAGNVEHHEMYRTFNC GVGMIIALPAPEVDKALALLNANGENAWKIGIIKASDSEQRVVIE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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