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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP864417NGFYeast1mgQuote
EP864417NGFE. coli1mgQuote
BP864417NGFBaculovirus200ugQuote
MP864417NGFMammalian Cell200ugQuote

Protein Information

SpeciesNeisseria meningitidis serogroup A / serotype 4A (strain Z2491)
UniProt IDQ9JVH4
Gene NamehisA; Locus:NMA0839
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFEQGARRLHLVDLNGAFAGVP QNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDVIIGTAAVKNPDFVREACK AFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDDGVNSIIYTDIGRDGMMSGVN IDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKRGVAGAITGRAIYEGSIDFAQAQQL ADSLD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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