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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase 2 (hisA2)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase 2 (hisA2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP702667Yeast1mgQuote
EP702667E. coli1mgQuote
BP702667Baculovirus200ugQuote
MP702667Mammalian Cell200ugQuote

Protein Information

SpeciesSilicibacter pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
UniProt IDQ5LR59
Gene NamehisA2; Locus:SPO2272
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase 2
Region Expressed1-247
Expression Tag6xHis
Purity>90%
AA SequenceMIIYPTMELQNGRCVTLERGRLDAPMLWHVDPFETVSGWASAGAEWMHLTDLDAVAGTSG NAELVEQIIRKAEIPVQLAGGMRSRERIEHWIDKGAGRIVIGTLAARDPELVKELAKRHP DQIVLSVDVWQGHVMTDGWRSQSAYTPEAFIDAFADVPFAAIVVTDIDSDVEEVEAKLGL ISGLAAHSRTPVIASGVVRGADDISRLAYVPNIAGALVGRALFRKSLSLEDALSIAASAH ERVAQFQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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