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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP689107Yeast1mgQuote
EP689107E. coli1mgQuote
BP689107Baculovirus200ugQuote
MP689107Mammalian Cell200ugQuote

Protein Information

SpeciesStaphylococcus epidermidis (strain ATCC 35984 / RP62A)
UniProt IDQ5HKP1
Gene NamehisA; Locus:SERP2302
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-234
Expression Tag6xHis
Purity>90%
AA SequenceMIDLWPAIDLINSTSVRLTEGKYDSKEKMEKSVEDSIRFYSQFKCVKRIHIVDLIGAKAK EVKEFDYIRSLRKLTTKPIEVGGGIRSKQTIENYIHSGIDYCIVGTKGIQDIEWLTHMTH QFPNKLYLSVDAFGEKIKINGWKEDAKLNLFDYVAKIEHLPLGGVIYTDISKDGKLSGPN FDLTGRLALYTSLPVIASGGIRHQEDLFRLESLNVHAAIVGKAAHLDEFWEGLS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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