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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP689650FPZYeast1mgQuote
EP689650FPZE. coli1mgQuote
BP689650FPZBaculovirus200ugQuote
MP689650FPZMammalian Cell200ugQuote

Protein Information

SpeciesSynechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
UniProt IDQ5MZR5
Gene NamehisA; Locus:syc2265_c
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-256
Expression Tag6xHis
Purity>90%
AA SequenceMDVIPAIDLLGGQCVRLFQGDYDQAEVYGKDPVGMALRWAEAGAQRLHLVDLDGAKEGSP VNAEAIATIAQRLSIPVQVGGGLRDRDTVARLLDSGVERAILGTVAVERPALVEALAGEF PGQIAVGIDARSGKVATRGWLEDSGLTAVALAQQMADLGACALICTDIGRDGTLQGPNLE ELRAIAAAVSIPVIASGGVGSLTDLLSLLPLEAQGVSGVIVGKALYTGAVDLQEALRAIG SGRWQDVAVDDSSRLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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