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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP691124SBFYeast1mgQuote
EP691124SBFE. coli1mgQuote
BP691124SBFBaculovirus200ugQuote
MP691124SBFMammalian Cell200ugQuote

Protein Information

SpeciesSalmonella choleraesuis (strain SC-B67)
UniProt IDQ57MR9
Gene NamehisA; Locus:SCH_2086
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMIIPALDLIDGTVVRLHQGDYARQREYGNDPLPRLQDYAAQGAGVLHLVDLTGAKDPAKR QIPLIKTLVAGVNVPVQVGGGVRTEEDVAALLKAGVARVVIGSTAVKSPDVVKGWFERFG AQALVLALDVRIDEHGNKQVAVSGWQENSGVSLEQLVETYLPVGLKHVLCTDISRDGTLA GSNVSLYEEVCARYPQIAFQSSGGIGNIDDIAALRGTGVRGVIVGRALLEGKFTVKEAIQ CWQNV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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