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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP664125Yeast1mgQuote
EP664125E. coli1mgQuote
BP664125Baculovirus200ugQuote
MP664125Mammalian Cell200ugQuote

Protein Information

SpeciesPelodictyon luteolum (strain DSM 273) (Chlorobium luteolum (strain DSM 273) )
UniProt IDQ3B287
Gene NamehisA; Locus:Plut_1690
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-256
Expression Tag6xHis
Purity>90%
AA SequenceMLIIPAIDIKDGKCVRLTRGDFSQQKIYLDNPLDMAIIWRKQNAKMLHIVDLDAALTGEM VNFERIRQIVEELDIPVQVGGGIRSADDVKRYLDMGVGRVVIGSAAVTNPELVRELMKTW RASKIVVGIDAVNGVPKIKGWTESANLKDFELAQRMKDMGIERIIYTDITRDGMLQGVGY ETTRRFAELAGMKVTASGGVTNSEDLRRLAGLQYCGVDSVIIGKAFYECNFPCQELWYNF EEGICLDHNFSTARKR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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