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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP707456MRUYeast1mgQuote
EP707456MRUE. coli1mgQuote
BP707456MRUBaculovirus200ugQuote
MP707456MRUMammalian Cell200ugQuote

Protein Information

SpeciesMethanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
UniProt IDQ58927
Gene NamehisA; Locus:MJ1532
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-237
Expression Tag6xHis
Purity>90%
AA SequenceMIIIPAVDLKDKKCVQLIQGDPNKKHLELNNPVEVAKKFVDEGAEYLHIIDLDAAFGTGN NRDVIKNIIKEVNVPVEVGGGIRNLEIAKELISLGVDRVIVGTKAILEPKFIDDLNKEIG KDKIVLAVECKEGKVVIKGWKEKVDKTPIEVIKEFEDKVGYILFTNVDVEGLLKGINVDI IKELIEKTDIPIIYSGGITTLEDIKALKELGIYGVVIGSALYKGLIDLKKALEIVKN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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