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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP614962RFJYeast1mgQuote
EP614962RFJE. coli1mgQuote
BP614962RFJBaculovirus200ugQuote
MP614962RFJMammalian Cell200ugQuote

Protein Information

SpeciesRoseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114) ) (Roseobacter denitrificans)
UniProt IDQ162Q2
Gene NamehisA; Locus:RD1_3560
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-239
Expression Tag6xHis
Purity>90%
AA SequenceMILYPAIDLKDGQAVRLVHGDMDRTTVFNDDPAAQARSFVAAGCTWLHLVDLNGAFAGEP VNAAPVEAILKACPVPAQLGGGIRDMATIERWLNKGLARVILGTVAVENPDLVREAARAF PDQVAVGIDARNGFVATKGWAEETDVQATDLAKSFEDAGVAAIIYTDILRDGAMGGPNTQ ATADLARAVSIPVIASGGVSSLDDLLALRDSGVISGAISGRALYDGAIDLAQALEALKT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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