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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (SPAC3F10.09)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (SPAC3F10.09) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP604568SXVYeast1mgQuote
EP604568SXVE. coli1mgQuote
BP604568SXVBaculovirus200ugQuote
MP604568SXVMammalian Cell200ugQuote

Protein Information

SpeciesSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
UniProt IDQ10184
Gene NameORFs:SPAC3F10.09
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-264
Expression Tag6xHis
Purity>90%
AA SequenceMEKSQHTLFRPCIDIHQGQVKQIVGGTLGNDADVKTNYVSLKPSSYYAELYKLNHLEGAH VIMLGPNCEEAAKTALHTWPGALQIGGGINISNAQNWLQEGASKVIVTSWLFPDAQFDLD RLKAISSKIGKDRLVVDVSCRRKDNRWFAAINKWQVMTAFELNEENLDLLSQYCSEFLIH AADVEGLCKGIDEDLVIKLGEWVKIPTTYAGGGRSIEDLDLVDKLSKGKVDLTIGSALDI FGGVLEFTRVVAWNRRFTPLPLQD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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