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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA)

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase (hisA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP640833Yeast1mgQuote
EP640833E. coli1mgQuote
BP640833Baculovirus200ugQuote
MP640833Mammalian Cell200ugQuote

Protein Information

SpeciesErythrobacter litoralis (strain HTCC2594)
UniProt IDQ2N8I7
Gene NamehisA; Locus:ELI_09560
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-242
Expression Tag6xHis
Purity>90%
AA SequenceMIVFPAIDLKGGEVVRLAEGDMDRATVYGDNPAAQAMLFAEAGAEHLHVVDLDGAFAGES RNREAVEAIVAEFPGYVQLGGGIRDATAVEGWFNLGVARIVIGSAALKDPDFVKEMAREW ENGIVVAVDARDGMIATEGWAEVSDVPVADMARRFEDAGVASLLFTDIGRDGLLKGCNID ATVELARQTDMPVIASGGVKGLDDIHVLSLHAHEGIEGVITGRALYEGRLDLAAAIAMGA RS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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