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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP501101FFUYeast1mgQuote
EP501101FFUE. coli1mgQuote
BP501101FFUBaculovirus200ugQuote
MP501101FFUMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas fluorescens (strain SBW25)
UniProt IDC3K6V2
Gene NamehisA; Locus:PFLU_0330
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMLIIPAIDLKDGACVRLRQGRMEDSTVFSDDPVSMAAKWVEGGCRRLHLVDLNGAFEGQP VNGEVVTAIAKRYPNLPIQIGGGIRSLETIEHYVKAGVSYVIIGTKAVKDPAFVAEACRA FPGKVIVGLDAKDGFVATDGWAEISTVQVIDLAKQFEADGVSAIVYTDIAKDGMMQGCNV PFTAALAAATKIPVIASGGIHNLGDIKSLLDAKAPGIIGAITGRAIYEGTLDVAEAQAYC DAYNG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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