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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP505093TOQYeast1mgQuote
EP505093TOQE. coli1mgQuote
BP505093TOQBaculovirus200ugQuote
MP505093TOQMammalian Cell200ugQuote

Protein Information

SpeciesTolumonas auensis (strain DSM 9187 / TA4)
UniProt IDC4LFI3
Gene NamehisA; Locus:Tola_1740
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMIIPAIDLIDGKVVRLYQGDYGQKTEYSADPQGRFDDYVAQGATQLHLVDLDGAKDSTKR QLTVIRKLLANTKAPVQIGGGVRTEQDVKDLLDAGANRVVIGSTAVKDPVTVAGWVEKYG ADKIVLALDVNIDAEGNRKIAVAGWQEDSGVTIEALLAHYLPVGLKHVLCTDISRDGTLQ GSNVALYRDLAAQFPQINWQASGGIGGIADIEALKGSGVGGVILGRSLLEGKFTVKEAIA CWQDA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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