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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP493342DULYeast1mgQuote
EP493342DULE. coli1mgQuote
BP493342DULBaculovirus200ugQuote
MP493342DULMammalian Cell200ugQuote

Protein Information

SpeciesClostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10)
UniProt IDB8I5V5
Gene NamehisA; Locus:Ccel_0387
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-236
Expression Tag6xHis
Purity>90%
AA SequenceMIIYPAIDIIDGKCVRLQQGSYSDVTVFGDSPVDMARKWESLGAGYLHVVDLDGARSGKS ENAEIIKQIAKTLKIPVQTGGGIRNLETVETYLSGGLSRIILGTSAVSNREMLISALKEY KGKIAVGIDAKDGKVAIHGWEKTSDYTAVEFAKEVESLGAKTIIYTDISRDGMLKGPNLQ AMKEMADSVSMDVIASGGVSRLKDIIDLKQTGVSGVIVGKAIYTGNVDLKEAILAI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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