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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP500129BQKYeast1mgQuote
EP500129BQKE. coli1mgQuote
BP500129BQKBaculovirus200ugQuote
MP500129BQKMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus (strain 03BB102)
UniProt IDC1EMQ6
Gene NamehisA; Locus:BCA_1464
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-239
Expression Tag6xHis
Purity>90%
AA SequenceMEIFPAIDLKEGRCVRLYQGEFSKETVMNEDPVAQAIIFEKFGAKRLHIVDLDGAVAGES LNLSVIERICKAVRIPVQVGGGIRSLVAVEKLFSVGVDKVILGTAALYDKTFLEEAVLLY KEKIIVGIDAKNGFVATRGWLDVSEISYIDLAKQMENIGVQTIVFTDISKDGTLAGPNIG QLELLQKSVAIRLIASGGVASIQDVKKLNDMNIYGVIIGKALYEKTIDLEEVLEVTKLC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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