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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP491216DJJYeast1mgQuote
EP491216DJJE. coli1mgQuote
BP491216DJJBaculovirus200ugQuote
MP491216DJJMammalian Cell200ugQuote

Protein Information

SpeciesDesulfitobacterium hafniense (strain DCB-2 / DSM 10664)
UniProt IDB8FP23
Gene NamehisA; Locus:Dhaf_1496
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-250
Expression Tag6xHis
Purity>90%
AA SequenceMRMRLFPAIDLKEGKAVRLLQGRMEDATVYGEQPVEVARKFKEQGADSLHVVDLDGAFAG KPVNDAVILKLIQSSGLRVQVGGGIRTLERIEELLKLGVERVILGTVAVRNPELVEKAVQ RFEEAVVIGIDAKDGLVAVQGWAEKTEIKALDLALRMKKVGVKHLVFTDISRDGMLQGPN IQSTVELARLSGLQVVASGGVSRLEDLRLLQEEANRGVSLEGAIVGKALYAGAFSLAEAL RVVGQRSEGK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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