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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP513430EJOYeast1mgQuote
EP513430EJOE. coli1mgQuote
BP513430EJOBaculovirus200ugQuote
MP513430EJOMammalian Cell200ugQuote

Protein Information

SpeciesEdwardsiella ictaluri (strain 93-146)
UniProt IDC5BG15
Gene NamehisA; Locus:NT01EI_2569
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMIIPALDFIDGSVVRLHQGDYGRQRDYDGDALARLLEYQRQGAAQLHLVDLSGARDPARR QLPMLTRLLRALSVPVQVGGGVRSAEDVATLLDAGATRVVVGSTAVRQPQEVARWFTRFG ADRLVLALDVRIAANGDKRVAVSGWQEDSGVTLEQTVAHFLPLGLRHVLCTDIACDGTLG GANVALYRQICPRYPQIEFQSSGGIGALQDITALRGSGVQGIIVGRALLEGKFSVKEAIA CWQNA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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