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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP519683MSRYeast1mgQuote
EP519683MSRE. coli1mgQuote
BP519683MSRBaculovirus200ugQuote
MP519683MSRMammalian Cell200ugQuote

Protein Information

SpeciesMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
UniProt IDO26931
Gene NamehisA; Locus:MTH_843
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-239
Expression Tag6xHis
Purity>90%
AA SequenceMLIIPAVDIKDGKCVQLVQGKPGTEQVVLDDPAGVAGRWESLGAETIHVVDLDGALGLEK NTGILKEITERVSVPLQIGGGIRSREYAGKLLDMGFERVILGTMAIENPAIVEELAGEYG SERIMVSLDSRDSRVVIRGWTEKVPFTAEEMARKFQERGAGSILFTNVDFEGLLSGFDLK PVSELVEAVEIPVIYSGGVSSLNDLRMLQGTGVMGVVIGSAIYRGLIDLTEALKYQDLK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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