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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP502300RKXYeast1mgQuote
EP502300RKXE. coli1mgQuote
BP502300RKXBaculovirus200ugQuote
MP502300RKXMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium sp. (strain NGR234)
UniProt IDC3MBC2
Gene NamehisA; Locus:NGR_c34010
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMILFPAIDLKDGQCVRLKLGDMDQATVYNPDPAAQARAFEEQGFEWLHVVDLNGAFAGET VNGAAVDAILKATGNPVQLGGGIRTLEHIENWLSHGLARVILGTVAVRDPALVIEACGKF PGRVAVGIDAKGGKVAVEGWAEASELGVIELAKKFEGAGVSAIIYTDIDRDGILTGINWD STLELANAVSIPVIASGGLASMDDIRRLAAPDAAKLEGAISGRALYDGRIDPKEALALIR AARKA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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