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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP521591DOCYeast1mgQuote
EP521591DOCE. coli1mgQuote
BP521591DOCBaculovirus200ugQuote
MP521591DOCMammalian Cell200ugQuote

Protein Information

SpeciesArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
UniProt IDO29276
Gene NamehisA; Locus:AF_0986
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-238
Expression Tag6xHis
Purity>90%
AA SequenceMFRVIPAIDLKDGKVVRLRQGKEDEVTFEATNPIEVAKQWVEIGAKVLHVIDLSGAFQGR LRHEEIIAEIAKMAEVQVGGGIRDFRVAERLFELGVDRVIFGTIAVERVEELREFAKKWK GRVMVAIDSKKGRVAVKGWKEVVELTPVQLAELYDDLDVSFLYTNIDVEGLVSGIERERI EEVVKSLRNPVYVAGGISSIEDIRFAKRVGAAGVVIGSALYTKKLKFEEAIKVEYEKV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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