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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP508719BWLYeast1mgQuote
EP508719BWLE. coli1mgQuote
BP508719BWLBaculovirus200ugQuote
MP508719BWLMammalian Cell200ugQuote

Protein Information

SpeciesBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
UniProt IDC0Z6P5
Gene NamehisA; Locus:BBR47_52670
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-249
Expression Tag6xHis
Purity>90%
AA SequenceMSFIVYPAIDIRGGKCVRLFQGDYGQETVYADSPLAMAKRWVEQGASWVHLVDLDGAKEG KPANAAIIKEIARSIPVPVQVGGGIRTEEQIADYLEAGVARVIVGTAAIEDEPFTKRILQ NDGDKIAIGLDCRNGLVATRGWLTTTDVQATELAKRLVTYGAETFIYTDIARDGTMTGPN VEEIAALAMATGKSVIASGGVSQLDDLLTLATHASDGVSGAIVGKALYTDAFTLEEALQR MEGRESYAG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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