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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP501580BQGYeast1mgQuote
EP501580BQGE. coli1mgQuote
BP501580BQGBaculovirus200ugQuote
MP501580BQGMammalian Cell200ugQuote

Protein Information

SpeciesBacillus anthracis (strain CDC 684 / NRRL 3495)
UniProt IDC3L9P8
Gene NamehisA; Locus:BAMEG_3166
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-239
Expression Tag6xHis
Purity>90%
AA SequenceMEIFPAIDLKEGRCVRLYQGEFSKETVMNEDPVAQAIIFEKFGAKRLHIVDLDGAVAGES LNLSVIERICKAVRIPVQVGGGIRSLVAVEKLFSVGVDKVILGTAALYDKTFLEEAVLLY KEKIIVGIDAKNGFVATRGWLDVSEISYIDLAKQMEKIGVQTIVFTDISKDGTLAGPNIE QLELLQKSVAIRLIASGGVASIQDVKKLNDMNIYGVIIGKALYEKTIDLEEVVEVTKLC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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