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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP501292DUEYeast1mgQuote
EP501292DUEE. coli1mgQuote
BP501292DUEBaculovirus200ugQuote
MP501292DUEMammalian Cell200ugQuote

Protein Information

SpeciesClostridium botulinum (strain 657 / Type Ba4)
UniProt IDC3KVX4
Gene NamehisA; Locus:CLJ_B1677
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-242
Expression Tag6xHis
Purity>90%
AA SequenceMIILPAIDLKEGKCVRLYQGDFKASKVVAEDPIEVALKFKENGAEYIHIVDLDGALTGQI KNLSIISSIIKTINIPVELGGGIRNLNTIDMLIDAGIERVILGTAALNNRGLVKEAVKKY DRKIAIGVDAKNEKVAINGWLNVSSTNYIDFAKEMENIGVRNIIFTDISKDGTLKGPNLN QLKKLNESVSCNIIASGGIKDIEDLKVIKEMDIYGAIVGKAIYSGNIDLNEAIKIINKES SR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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