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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP503358BQFYeast1mgQuote
EP503358BQFE. coli1mgQuote
BP503358BQFBaculovirus200ugQuote
MP503358BQFMammalian Cell200ugQuote

Protein Information

SpeciesBacillus anthracis (strain A0248)
UniProt IDC3P505
Gene NamehisA; Locus:BAA_1496
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-239
Expression Tag6xHis
Purity>90%
AA SequenceMEIFPAIDLKEGRCVRLYQGEFSKETVMNEDPVAQAIIFEKFGAKRLHIVDLDGAVAGES LNLSVIERICKAVRIPVQVGGGIRSLVAVEKLFSVGVDKVILGTAALYDKTFLEEAVLLY KEKIIVGIDAKNGFVATRGWLDVSEISYIDLAKQMEKIGVQTIVFTDISKDGTLAGPNIE QLELLQKSVAIRLIASGGVASIQDVKKLNDMNIYGVIIGKALYEKTIDLEEVVEVTKLC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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