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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP512958DJQYeast1mgQuote
EP512958DJQE. coli1mgQuote
BP512958DJQBaculovirus200ugQuote
MP512958DJQMammalian Cell200ugQuote

Protein Information

SpeciesDesulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763)
UniProt IDC6BSE3
Gene NamehisA; Locus:Desal_1557
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-242
Expression Tag6xHis
Purity>90%
AA SequenceMIVFPAVDIKDGVCVRLSQGQADAVTVFSSDPVAQAKYWETQGARYLHVVDLDGAFSGMP KNFELIRDICSQLSIPVQLGGGIRDIETASKYLEAGVRRLIIGTMALEDEETFAELCAKF PGQIGVSLDAVNGQLKSRGWVEDAGITVFDIIPRIEAAGAAFIIYTDISRDGMQTGVNVM ALSELCSKTKLPVIAAGGVATLEDVINLQPLVSKGLEGAVSGQAIYTGTLNFAEAMEWIE NN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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