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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP492872HUEYeast1mgQuote
EP492872HUEE. coli1mgQuote
BP492872HUEBaculovirus200ugQuote
MP492872HUEMammalian Cell200ugQuote

Protein Information

SpeciesHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
UniProt IDB8D115
Gene NamehisA; Locus:Hore_02230
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-237
Expression Tag6xHis
Purity>90%
AA SequenceMEVIPAVDIKDGSCVRLKKGDFNKRRVYSTSPVDVALYWEKHGASRLHIVDLDGAKSGWP THLKTIREIALRVNIPLQVGGGIRSLKVIKKYLDSGVDRIILGTVALKNPELVKRALDNF GSNRIVVGVDARGGKVATEGWLKTSQVTVEDIISEMEEVGVKTFIYTDINRDGMLKGPDI EGIKRVLKSTKARIIASGGISSRQDLINLKAIGIKAAIVGKALYEGNLPLEVLNQYP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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