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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP490908SYWYeast1mgQuote
EP490908SYWE. coli1mgQuote
BP490908SYWBaculovirus200ugQuote
MP490908SYWMammalian Cell200ugQuote

Protein Information

SpeciesShewanella piezotolerans (strain WP3 / JCM 13877)
UniProt IDB8CR53
Gene NamehisA; Locus:swp_3007
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-245
Expression Tag6xHis
Purity>90%
AA SequenceMIIPAIDLIEGQVVRLYQGDYNQKTTFNLSPLEQLQSYQEQGADLLHIVDLTGAKDPMKR QTQLIASLVRELDTPIQVGGGVRTEAQLSELLEIGVSRVVIGSLAVKEPELVKSWFVKYG SDAICLALDVNINDQGEKIVAVSGWQSGGGKTLESLVAEFSTVGLKHALVTDISRDGTLK GANTELYQEISTAYPEINWQASGGIATLADVNAVKESGANGIIIGKALLINQFTVEEAIT CWPNA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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