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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP492999BSUYeast1mgQuote
EP492999BSUE. coli1mgQuote
BP492999BSUBaculovirus200ugQuote
MP492999BSUMammalian Cell200ugQuote

Protein Information

SpeciesBifidobacterium animalis subsp. lactis (strain AD011)
UniProt IDB8DSW1
Gene NamehisA; Locus:BLA_0798
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-242
Expression Tag6xHis
Purity>90%
AA SequenceMSLTLLPAVDVRDGKAVRLRQGESGSETDYGSPLDAARTWVDAGAQWIHLVDLDAAFGTG DNRAQLREIVRQLGDKVNIEMSGGVRDDASLDAALDAGAARVNIGTAALENPEWTARVID KYGDKVAVGLDVRGHTLAARGWVKEGGGLFETMRFLDDAGCSRYVVTDVTRDGMMSGPNI DLLREVAERTDARVTASGGISKLDDLRAIKELLPLGVDSAILGKSLYAGAFTLEEALAVA NA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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