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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP490416DJRYeast1mgQuote
EP490416DJRE. coli1mgQuote
BP490416DJRBaculovirus200ugQuote
MP490416DJRMammalian Cell200ugQuote

Protein Information

SpeciesDesulfovibrio vulgaris (strain Miyazaki F / DSM 19637)
UniProt IDB8DNB4
Gene NamehisA; Locus:DvMF_3120
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-243
Expression Tag6xHis
Purity>90%
AA SequenceMILFPAVDIKDGRCVRLRQGRADAETVFSDDPAAMARHWQDQGAKWLHVIDLDGAFSGMP ANFELIRRICADLSVPVQLGGGIRDEATAKAYLDAGVERLIIGTVALEEPDLYARLCATF PGRIGVSLDAEGGRLKTKGWVADSGLTVDDVLPRLLAAGTAFVIYTDIDRDGMQTGVNLS ALERLAGMCPVPVIAAGGVATLEDVRALYPMTLTSSVEGAITGRAIYTGTLDLHAAMEWI AAQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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