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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP489714BWYYeast1mgQuote
EP489714BWYE. coli1mgQuote
BP489714BWYBaculovirus200ugQuote
MP489714BWYMammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Acyrthosiphon pisum (strain 5A)
UniProt IDB8D8Q6
Gene NamehisA; Locus:BUAP5A_102
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-246
Expression Tag6xHis
Purity>90%
AA SequenceMIIPAFDLINGRTVRLYQGDYSNQKNYNVNLFNSLEVYKSKGIEIVHLVDLDGAKNSANR QIELFKKIVSHTTVPVQVGGGIRTTKDISTLLDLGVKRVVIGSSIVSNKKQVKQWLNFYG PDAIVLALDVHVDGSNKKEISIDGWQKKTNFILEEIIEYFLSSGLKHVLCTDISRDGTLL GPNFKLYKEICSNFKNINFQASGGVASLQDIIFLKKTGVKSIIIGRSLLEKKFTIEEAVK CWQRES
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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