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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP489044BXAYeast1mgQuote
EP489044BXAE. coli1mgQuote
BP489044BXABaculovirus200ugQuote
MP489044BXAMammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Acyrthosiphon pisum (strain Tuc7)
UniProt IDB8D710
Gene NamehisA; Locus:BUAPTUC7_103
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-246
Expression Tag6xHis
Purity>90%
AA SequenceMIIPAFDLINGRTVRLYQGDYSNQKNYNVNLFNSLEVYKSKGIEIVHLVDLDGAKNSANR QIELLKKIVSHTTVPVQVGGGIRTTKDISTLLDLGVKRVVIGSSIVSNKKQVKQWLNFYG PDAIVLALDVHVDSSNKKEISIDGWQKKTNFILEEIIEYFLSSGLKHVLCTDISRDGTLL GPNFKLYKEICSNFKNINFQASGGVASLQDIIFLKKTGVKSIIIGRSLLEKKFTIEEAVK CWQRES
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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