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Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase

Recombinant 1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP392620PZJYeast1mgQuote
EP392620PZJE. coli1mgQuote
BP392620PZJBaculovirus200ugQuote
MP392620PZJMammalian Cell200ugQuote

Protein Information

SpeciesProsthecochloris vibrioformis (strain DSM 265) (Chlorobium vibrioforme subsp. thiosulfatophilum (strain DSM 265) ) (Chlorobium phaeovibrioides (strain DSM 265) )
UniProt IDA4SG77
Gene NamehisA; Locus:Cvib_1475
Protein Name1- (5-phosphoribosyl) -5-[ (5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase
Region Expressed1-256
Expression Tag6xHis
Purity>90%
AA SequenceMLIIPAIDIKDGKCVRLTRGDFSQQKIYLDNPLDMAIIWRKQNAKMLHIVDLDAALTGEM VNFDIIANIVAELDIPVQIGGGIRSIDAVKRYLDMGVGRVVIGSAAVTDPELVGELMKHW PASKIVVGIDAENGVPKIKGWTESSGMQDYELALRMKDMGVERIVYTDISRDGMMQGFGY ESTKRFAQKAGLKVTASGGVTNSEDLQRLATLRPYGVDSVIIGKAFYECNFPCQELWYNF EKGICLDQNFSTARRK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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