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Recombinant Phospholipase A1-IIgamma (DSEL)

Recombinant Phospholipase A1-IIgamma (DSEL) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP529871DOAYeast1mgQuote
EP529871DOAE. coli1mgQuote
BP529871DOABaculovirus200ugQuote
MP529871DOAMammalian Cell200ugQuote

Protein Information

SpeciesArabidopsis thaliana (Mouse-ear cress)
UniProt IDO49523
Gene NameDSEL; Locus:At4g18550; ORFs:F28J12.210
Protein NamePhospholipase A1-IIgamma
Region Expressed1-419
Expression Tag6xHis
Purity>90%
AA SequenceMKRKKKEEEEEKLIVTREFAKRWRDLSGQNHWKGMLQPLDQDLREYIIHYGEMAQAGYDT FNINTESQFAGASIYSRKDFFAKVGLEIAHPYTKYKVTKFIYATSDIHVPESFLLFPISR EGWSKESNWMGYVAVTDDQGTALLGRRDIVVSWRGSVQPLEWVEDFEFGLVNAIKIFGER NDQVQIHQGWYSIYMSQDERSPFTKTNARDQVLREVGRLLEKYKDEEVSITICGHSLGAA LATLSATDIVANGYNRPKSRPDKSCPVTAFVFASPRVGDSDFRKLFSGLEDIRVLRTRNL PDVIPIYPPIGYSEVGDEFPIDTRKSPYMKSPGNLATFHCLEGYLHGVAGTQGTNKADLF RLDVERAIGLVNKSVDGLKDECMVPGKWRVLKNKGMAQQDDGSWELVDHEIDDNEDLDF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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