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Recombinant 6-phosphofructokinase (pfkA)

Recombinant 6-phosphofructokinase (pfkA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP017822SKYYeast1mgQuote
EP017822SKYE. coli1mgQuote
BP017822SKYBaculovirus200ugQuote
MP017822SKYMammalian Cell200ugQuote

Protein Information

SpeciesStaphylococcus aureus (strain N315)
UniProt IDP99165
Gene NamepfkA; aka: pfk; Locus:SA1521
Protein Name6-phosphofructokinase
Region Expressed1-322
Expression Tag6xHis
Purity>90%
AA SequenceMKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGLLNDDIHKLELGSVGDT IQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGGDGSYRGAQRISEECKEIQ TIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTASSHARTFIIEAMGRDCGDLAL WAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHSIVLVAEGCMTAQDCQKELSQYI NVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDLLMQGETAKGVGIKNNKIVATSFDE IFDGKDHKFDYSLYELANKLSI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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