Request QuoteCatalog Number: xP017822DKKSize: 0.2-1mg

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Recombinant 6-phosphofructokinase (pfkA)

Recombinant 6-phosphofructokinase (pfkA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP017822DKKYeast1mgQuote
EP017822DKKE. coli1mgQuote
BP017822DKKBaculovirus200ugQuote
MP017822DKKMammalian Cell200ugQuote

Protein Information

SpeciesDictyostelium discoideum (Slime mold)
UniProt IDP90521
Gene NamepfkA; aka: pfk; ORFs:DDB_G0274111
Protein Name6-phosphofructokinase
Region Expressed1-834
Expression Tag6xHis
Purity>90%
AA SequenceMTTTSKIINDGEGEDVKGNKNINKKSLIDENRLDEKKDLLSDKVESKTHCSVKRMAVLTS GGDSSGMNPAIRAFARQVMLKGAKVFAVREGYNGLVNDSIVPLNWGSVAGIISRGGTIIG TARSAEFRTREGRKRAVFNLVKNRIDNLLVIGGDGSLTGANLLRTEWCSLLEELVKDGKL TLDVMEHFPILSIAGIVGSIDNDMCGTDLTVGADTATKRILEAIDSILSTAVSHQRSFVI EVMGRNCGWLALASGVATGADYILIPESPPDDGWEQTMADNLERGRLSGRRCSLVIVSEG AIDRQGKPITSAYVRQFLEDKGHDARITILGHVQRGGTPTFLDRYIATRMGIEAANYFYD STIEQLKQPVLIGMSGMDTIRSPLMECVQKTQSIASLIKERRFNEVVDVRGGMFKEFYEI FIACSNLHRRKVESKGMGVLILHSGGPSPGMNPCVRAFTRLGIDHGYTMYGCFNGFGGLA LGEIEQLHWMTVNGWSVMGGAELGTNRSIPNDSNIEAIIATLERFKINAILMFGGFNGYL GIAKLYEYREKYQQLKRISIIGAPGTIANNVPGTNISIGSDTSLNNTLDALDKIKQSAVA SRRLFVVEVMGAHCGYLAAMSSLTSGAERSYIMERGITLNTLTKDLEMFVERFKREHRIG LIIKSELASNTYSTHFIYSLFKEEGKHLFDVRESILGHLQQGGTPSAIDRIFSTRLMNHY YQFLENDLKEHGHLQMNGCIGFIDGGIHYTPMQEMIEEMSDKFRRPRSQWWMDLVETSQN ISVFPLDDPSSTNFEGCNSNLSEQDRPIKKSDISSPTSYSQKTFDPNVNPQFTL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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